王际睿,博士,教授 Jirui Wang, Ph.D.,Professor
2022世界杯卡塔尔下注院长 Dean, College of Agronomy
email：
jirui.wang@gmail.com;
isphsc@sicau.edu.cn.
长江学者、国家973计划项目（青年）首席科学家、四川省学术技术带头人、四川省突出贡献专家。从事作物种子发育-萌发与谷物品质研究：鉴定作物种子发育相关关键基因PHS-3D、PGS1等，解析穗发芽与老化引起作物品质劣化的机制，从麦类种质资源中挖掘优异基因并用于抗性育种。在Mol. Plant, Plant Biotechnol. J., New Phyto., PNAS, Cell等杂志发表论文70余篇；获四川省科技进步一等奖2项、四川省青年科技奖；获授权发明专利2件、制定地方标准6项、参与作物新品种选育2个。
教育经历Education：
Ph.D.作物遗传育种（Plant Breeding）,四川农业大学（Sichuan Agricultural University）
MSc作物遗传育种（Plant Breeding）,四川农业大学（Sichuan Agricultural University）
BSc生化与分子生物学（Biochemistry & Molecular biology）,兰州大学（LanzhouUniversity）
学术与职业经历Academic & Professional Experience：
四川农业大学小麦研究所（Triticeae Research Institute, Sichuan Agricultural University, Chengdu Sichuan, 2008-Now）
The main works in our group are 1) characterization of genes related to seed development and germination in cereals, 2) PHS resistant wheat breeding, and 3) wheat quality enhancement.
I worked with Drs. Mingcheng Luo and Jan Dvorak during 2011-2013 as postdoc fellow.Westudied the adaptive evolution of the D genome donor of hexaploid wheat,Ae.tauschii. This project was supported by NSF and USDA.
加拿大农业部渥太华研究中心（ECORC-AAFC, Ottawa, ON, Canada, Jan 2007- Feb 2008）.
A project about characterizing seed genes with specific expression levels in Fusarium infected wheat was carried out.
西南作物基因资源与遗传改良教育部重点实验室主任（Director of theMinistry of Education Key Laboratory for Crop Genetic Resources and Improvement in Southwest China,2020-now）
食品安全检测（四川省）重点实验室学术委员会委员（2017-now）
固态酿造技术（四川省）创新中心专家委员会委员（2022-now）
国际谷物穗发芽大会主委会委员/主席（Committee member of the 14thand 15thInternational Symposium of Pre-harvest sprouting on Cereals）
国际植物休眠会议组委会委员（Committee member of the 7thInternational Plant Dormancy Symposium）
<种子科学研究>特邀编辑（Guest Editor of<Seed Science Research>, 2019-2021）
<农学>编辑（Editor of <Agronomy>, 2021-now）
<种子>编辑（Editor of <Seeds>, 2022-now）
授课课程Courses：
种子生物学Seed Biology,种子科学与技术本科生课程秋季学期
种子科学研究进展Advance in Seed Science,作物遗传育种博士生课程秋季学期
研究及招生方向Current Research：
结合多组学数据分析与分子生物学技术，对作物种子发育-萌发关键基因进行鉴定，通过遗传分析及基因编辑等策略鉴定其功能并构建调控网络，达到解析作物种子大小、萌发、寿命等重要性状调控机制的目的。
作物籽粒发育过程中积累的淀粉、蛋白等物质特性决定作物品质；穗发芽(PHS)、陈化等负面影响作物品质。解析品质形成与保持机理，发掘优质性状形成、穗发芽抗性、延缓陈化的关键基因，并解析其内源、外源调控机制，为高产优质作物生物育种奠定基础。
The goal of our group is to understand the environment affection, epigenetic mechanisms, and gene networks that control seed development, dormancy, and germination in cereals. The environment, epigenetic mechanisms, and gene networks affect many essential processes of seed, including embryo development, grain filling, maturation, longevity, and germination. Many of them are necessary for crop production, such as yield, quality, nutrition, and Pre-harvest sprouting (PHS) resistance. PHS is defined as the germination of grains in the ears before harvest, which causes a decrease in end-use quality due to starch and protein degradation. PHS is considered a worldwide disaster that reduces the production of cereals and damages their quality. In recent years, our group is working on the characterization of PHS resistant genes and selecting elite breeding materials.
部分研究工作Selected works:
Aegilops tauschii single nucleotide polymorphisms shed light on the origins of wheat D-genome genetic diversity and pinpoint the geographic origin of hexaploid wheat.New Phytologist,2013, 198: 925-937
Hexaploid wheat (Triticum aestivum, genomes AABBDD) originated by hybridization of tetraploidTriticumturgidum(genomes AABB) withAegilops tauschii(genomes DD). Genetic relationships between A. tauschii and the wheat D genome are of central importance for the understanding of the wheat origin and subsequent evolution. Genetic relationships among 477A. tauschiiand wheat accessions were studied with the A. tauschii 10K Infinium single nucleotide polymorphism (SNP) array.Aegilops tauschiiconsists of two lineages (designated 1 and 2) having little genetic contact. Each lineage consists of two closely related sublineages. A population within lineage 2 in the southwestern and southern Caspian appears to be the main source of the wheat D genome. Lineage 1 contributed as little as 0.8% of the wheat D genome. Triticum aestivum is subdivided into the western and Far Eastern populations. The Far Eastern population conserved the genetic make-up of the nascent T. aestivum more than the western population. In wheat, diversity is high in chromosomes 1D and 2D, and it correlates in all wheat D-genome and A. tauschii chromosomes with recombination rates. Gene flow fromA. tauschiiwas an important source of wheat genetic diversity and shaped its distribution along the D-genome chromosomes.
Uncovering the dispersion history, adaptive evolution and selection of wheat in China.Plant Biotechnology Journal, 2018, 16: 280-291
Wheat was introduced to China approximately 4500 years ago, where it adapted over a span of time to various environments in agro-ecological growing zones. We investigated 717 Chinese and 14 Iranian/Turkish geographically diverse, locally adapted wheat landraces with 27,933 DArTseq (for 717 landraces) and 312,831 Wheat660K (for a subset of 285 landraces) markers. This study highlights the adaptive evolutionary history of wheat cultivation in China. Environmental stresses and independent selection efforts have resulted in considerable genome-wide divergence at the population level in Chinese wheat landraces. In total, 148 regions of the wheat genome show signs of selection in at least one geographic area. Our data show adaptive events across geographic areas, from the xeric northwest to the mesic south, along and among homoeologous chromosomes, with fewer variations in the D genome than in the A and B genomes. Multiple variations in interdependent functional genes, such as regulatory and metabolic genes controlling germination and flowering time were characterized, showing clear allelic frequency changes corresponding to the dispersion of wheat in China. Population structure and selection data reveal that Chinese wheat spread from the northwestern Caspian Sea region to south China, adapting during its agricultural trajectory to increasingly mesic and warm climatic areas.
WheatGmap: A Comprehensive Platform for Wheat Gene Mapping and Genomic Studies.Molecular Plant,2021, 14: 187-190
Gene mapping of mutations is a critical step for new gene discovery and functional analysis. However, this step has been limited by the discovery of very dense sets of informative markers. Here, we report a platform, Wheat Genomic Map (WheatGmap, https://www.wheatgmap.org), for the fast and cost-effective mapping of genes in wheat. The main application of this platform is the use of several statistical models enabling researchers to simply and flexibly conduct bulked segregant analysis (BSA)-based causal variation mining and gene mapping. A significant feature of the platform is that large-scale genomic variant data can be used as a resource to filter non-causal SNPs or non-validated candidate genes. Moreover, users can share their genomics data of genetic information and annotated phenotypes via WheatGmap. In the current version (WheatGmap 1.0), more than 3,500 next-generation sequencing datasets, including whole-exome sequencing (WES), whole-genome sequencing (WGS), and RNA-seq datasets from public resources, were processed with our standard pipeline. These online tools and genomics data could become an easily applicable resource for gene mapping in a complex wheat genome background.
Myb10-DconfersPHS-3Dresistance to pre-harvest sprouting by regulating NCED in ABA biosynthesis pathway of wheat.New Phytologist2021,230: 1940-1952
Pre-harvest sprouting (PHS), the germination of grain before harvest, is a serious problem resulting in wheat yield and quality losses. Here, we mapped the PHS resistance gene PHS-3D from synthetic hexaploid wheat to a 2.4 Mb presence-absence variation (PAV) region and found that its resistance effect was attributed to the pleiotropicMyb10-Dby integrated omics and functional analyses. Three haplotypes were detected in this PAV region among 262 worldwide wheat lines and 16 Aegilops tauschii, and the germination percentages of wheat lines containingMyb10-Dwas approximately 40% lower than that of the other lines. Transcriptome and metabolome profiling indicated that Myb10-D affected the transcription of genes in both the flavonoid and ABA biosynthesis pathways, which resulted in increases in flavonoids and ABA in transgenic wheat lines. Myb10-D activates NCED by biding the secondary wall MYB-responsive element (SMRE) to promote ABA biosynthesis in early wheat seed development stages. We revealed that the newly discovered function ofMyb10-Dconfers PHS resistance by enhancing ABA biosynthesis to delay germination in wheat. The PAV harboringMyb10-Dassociated with grain color and PHS will be useful for understanding and selecting white-grained PHS resistant wheat cultivars.
The PGS1 basic helix-loop-helix (bHLH) protein regulates Fl3 to impact seed growth and grain yield in cereals.Plant Biotechnology Journal2022,online
Plant transcription factors, such as basic helix-loop-helix (bHLH) and AT-rich zinc-binding proteins (PLATZ), play criticalrolesin regulating the expression of developmental genesin cereals. We identified the bHLH protein TaPGS1 (T. aestivumPositive Regulator of Grain Size 1) specifically expressed in the seeds at 5–20 days post-anthesis in wheat.TaPGS1was ectopically overexpressed (OE) in wheat and rice, leading to increased grain weight (up to 13.81% in wheat and 18.55% in rice lines) and grain size. Carbohydrate and total protein levels also increased. Scanning electron microscopy results indicated that the starch granules in the endosperm ofTaPGS1OE wheat and rice lines were smaller and tightly embedded in a proteinaceous matrix. Furthermore,TaPGS1 was bound directly to the E-box motif at the promoter of the PLATZ transcription factor genesTaFl3andOsFl3and positively regulated their expression in wheat and rice. In rice, theOsFl3CRISPR/Cas9 knockout lines showed reduced average thousand-grain weight, grain width, and grain length in rice. Our results reveal thatTaPGS1functions as a valuable trait-associated gene for improving cereal grain yield.
Lab News:
20220322:郭晓江等论文“The PGS1 basic helix-loop-helix (bHLH) protein regulates Fl3 to impact seed growth and grain yield in cereals”在Plant Biotechnology Journal在线发表（DOI: 10.1111/pbi.13809）。
20220224:何雨等论文“Temporal transcriptomes unravel the effects of heat stress on seed germination during wheat grain filling”在Journal of Agronomy And Crop Science在线发表（DOI: 10.1111/jac.12586）。
20211208：唐豪等论文“Genome-wide association studies of grain black point resistance in Chinese wheat landraces”在Plant Disease在线发表（DOI: 10.1094/PDIS-09-21-1898-RE）。
20210809-13：参加13届国际种子科学大会ISSS-2020（Online,UK）。
20210326:肖春生等论文“Characterization and expression quantitative trait loci analysis ofTaABI4, a pre-harvest sprouting related gene in wheat”在Seed Science Research发表（DOI: 10.1017/S0960258521000015）。
20210302:郎静等论文“Myb10-DconfersPHS-3Dresistance to pre-harvest sprouting by regulating NCED in ABA biosynthesis pathway of wheat”在New Phytologist发表（DOI: 10.1111/nph.17312）。
20210203:张立超、董春豪、陈中序等论文“WheatGmap: A Comprehensive Platform for Wheat Gene Mapping and Genomic Studies”在MolecularPlant发表（DOI: 10.1016/j.molp.2020.11.018）。
20201108:符语昕等论文“Identification and Characterization of PLATZ Transcription Factors in Wheat”在IJMS发表。
20190829：杨剑等论文“Identification of qPHS.sicau-1B and qPHS.sicau-3Dfrom synthetic wheat for pre-harvest sprouting resistancewheat improvement”在Molecular Breeding发表。
20190730-0802：第十四届“国际谷物穗发芽大会”顺利召开（isphsc.csp.escience.cn）。大会共吸引到了来自中国、美国、日本、澳大利亚、英国等国家80余名行业内专家学者到场，围绕“种子休眠与发芽”、“淀粉酶与穗发芽”、“穗发芽分子遗传机制”、“穗发芽抗性育种”四个专题展开了深入的交流与讨论。刘宇娇、黄雯做会议报告。王际睿当选为第十五届大会（日本筑波，2023）主席。
感谢课题组及小麦研究所、四川农业大学、四川省农科院等老师、同学们对会议的支持，同时感谢罗氏、博奥晶典、库蓝科技、瀚辰光翼、慧诺瑞德、肖邦技术等公司的协助。
20181117：科技部验收王际睿主持的国家973计划项目“芽麦对品质的影响及抗穗发芽小麦材料全基因组选育”。
20180822：周勇等论文“白皮小麦抗穗发芽资源评价及抗性候选位点关联分析”在麦类作物学报发表。
20180121：周勇等论文“Uncovering the Dispersion History, Adaptive Evolution and Selection of Wheat in China”在Plant Biotechnology Journal发表。
20171214-15：王际睿、程梦萍、陈中旭参加2017年度国家超级计算广州中心“天河二号”超算应用大会，“作物基因组大数据分析”工作获得2017年度优秀应用奖。
20170909-22：王际睿、陈中旭参加12届国际种子科学大会ISSS（Monterey, CA），并做“Characterization of pre-harvest sprouting resistance genes in a large germplasm collection of Chinese wheat landraces&Aegilops tauschii”报告；访问UC Davis。
20170822：郭晓江参加第18届全国植物基因组学大会（兰州），获得优秀墙报奖。
20170715-29：王际睿参加“中-澳青年科学家交流项目”（Australia China Young Scientists Exchange Program）访问澳大利亚CSIRO_Canberra, CSIRO_Brisbane, University of Queensland, Murdoch University, University of West Australia，进行学术交流与报告。
20170702：郭晓江论文“Global identification, structural analysis and expression characterization of bHLH transcription factors in wheat”在BMC Plant Biology发表。
20170610：陈学伟团队完成的“A natural allele of a transcription factor in rice confers broad-spectrum blast"在Cell发表。王际睿、程梦萍、陈中旭进行了该论文大数据分析部分工作。
20170514：王际睿参加第五届全国种子科学与技术学术研讨会（桂林）并做学术报告。
20170429：周勇等论文“Genome-Wide Association Study for Pre-harvest Sprouting Resistance in a Large Germplasm Collection of Chinese Wheat Landraces”在Frontiers in Plant Science发表。
20170228：陈真勇等论文“Identification and positional distribution analysis of transcription factor binding sites for genes from the wheat fl-cDNA sequnces”在Bioscience, Biotechnology, and Biochemistry发表。
20170126：周科等论文“Sequence analysis and expression profiles of TaABI5, a pre-harvest sprouting resistance gene in wheat”在Gene & Geno发表。
20161227：刘宇娇等论文“Conferring resistance to pre-harvest sprouting in durum wheat by a QTL identified in Triticum spelta”在Euphytica发表。
20160918：王际睿、周勇参加第13届国际谷物穗发芽会议（澳大利亚-佩斯）并分别做主题报告，同时成功申请承办下一届会议。
20160814：王际睿、刘宇娇、谭超参加第7届世界作物学大会（北京），期间王际睿做主题报告。
20160315：陈中旭等论文“SNP mining in functional genes from nonmodel species by next-generation sequencing: ... in wheat”在BMRI发表。
20151112：王际睿、杨剑、周勇参加第7届全国小麦遗传育种研讨会（河南-郑州），周勇获得优秀博士研究生墙报奖。
20150818：王际睿、杨剑、周勇参加第6届中国小麦基因组与分子育种大会（陕西-杨凌），王际睿做大会特邀报告。
20150302：陈真勇、郭晓江等论文“Genome-wide characterization of developmental stage- and tissue-specific transcription factors in wheat”在BMC Genomics发表。
20140915-19：王际睿参加11届国际种子科学大会ISSS（Changsha, China）。
20140408：杨剑等论文“Molecular characterization of high pI α-amylase and its expression QTL analysis in synthetic wheat RILs”被Molecular Breeding接收。
Lab Members:
董慧雪博士，讲师，硕士生导师
Huixue Dong,PhD, Institute of Crop Science, CAAS
研究及招生方向Current Research:作物功能基因组&遗传改良Crop Functional Genomics & Genetics Improvement
程梦萍，讲师
Mengping Cheng, BSc, Chengdu Neusoft University
研究方向Research:作物生物信息学&数据发掘Crop Bioinformatics & Data Mining
李茂莲，讲师
Maolian Li, MSc, South West University
研究方向Research:育种技术-编辑&转化Breeding technology - Editing & Transformation
Former Research Assistant
王早霞(Zaoxia Wang), 2013-2016;龙茜(Xi Long), 2013-2014;李净琼(Jingqiang Li), 2009-2013
Ph.D. Students
陈真勇(Zhenyong Chen) 2013-2015
杨剑(Jian Yang) 2012-2016
周勇(Yong Zhou) 2010-2013-2017
刘宇娇(Yujiao Liu) 2014-2018;联合培养:2014.09-2015.09, AAFC-Ottawa加拿大
陈中旭(Zhongxu Chen) 2012-2015-2021;联合培养:2016.04-2017.09, UC Davis美国
张琴(Qin Zhang) 2013-2016-2021;联合培养:2018.11-2021.2,CSIRO_Canberra澳大利亚
桂李暄(Lixuan Gui) 2016-now
郭晓江(Xiaojiang Guo) 2014-2017-2022;联合培养:2018.12-2021.12, UC Davis美国
Iqbal Hussain 2017-2018
唐豪(Tang Hao) 2015-2018-now
谭超(Tan Chao) 2015-2018-now
郎静(Jin Lang) 2016-2019-now)
符语昕(Yuxin Fu) 2017-2020-now
何雨(Yu He) 2020-now
蒲茜(Xi Pu) 2021-now
Ummar Ali2021-now
MSc Students
刘昆(Kun Liu) 2011-2014
朱松(Song Zhu) 2013-2016
Kwame Obeng Dankwa 2015-2017
肖春生(Chunsheng Xiao) 2016-2020;联合培养:2019.11-2020.04,Murdoch澳大利亚
杨力生(Lisheng Yang) 2016-2019
黄雯(Wen Huang) 2017-2020
王祥向(Xiangxiang Wang) 2017-2020
邓敏(Min Deng) 2017-2020
张承碧(Chengbi Zhang) 2018-2021
李令川(Lingchuan Li) 2019-2022
梁王壮(Wangzhuang Liang) 2020-now
李之恒(Zhiheng Li) 2020-now
杨珞(Luo Yang) 2021-now
林媛媛(Yuanyuan Lin) 2021-now
张文鹏(Wenpeng Zhang) 2021-now
Undergraduate Students
2022:古敬(Jin Gu),刘佳芸(Jiayun Liu),曹馨引(Xinyin Cao),王铭尉(Mingwei Wang)
2021:唐雅楠(Yanan Tang),陈宏宇(Hongyu Chen),许力木(Limu Xu),刘禄宏(Luhong Liu),徐茂祥(Maoxiang Xu)
2019:宋秋池(Qiuci Song)
2017:吴晓葭(Xiaojia Wu)
2016:郭世宽(Shikuan Guo),曾小玉(Xiaoyu Zeng),李晶(Jin Li),端木笑盈(Xiaoying Duanmu),王冉(RanWang),张承碧(Chenbi Zhang),李承志(Chengzhi Li)
2015:马欣源(Xinyuan Ma)
2014:符语昕(Yu-Xin Fu)
2013:毛瑞文(Ruiwen Mao),谢娇(Jiao Xie)
2012:余璨(Can Yu),陈文帅(WenShuai Chen),肖春生(Chunsheng Xiao),陈丽娜(Lina Chen)
2011:周科(Ke Zhou),段杰(Jie Duan),郎静(Jing Lang)
